Journal: Nutrients

Article Title: Tryptophan Attenuates Chronic Restraint Stress-Induced Intestinal Injury Through Modulation of Intestinal Barrier Integrity and Gut Microbiota Homeostasis

doi: 10.3390/nu17060975

Figure Lengend Snippet: Figure 3. Tryptophan alleviates CRS-induced intestine leakage and epithelial apoptosis. (A) Serum intestinal fatty acid-binding protein content by ELISA; (B) FITC-dextran content in serum; (C) TUNEL staining plots; (D) TUNEL-positive cell counts; different letters denote significant differences between groups, p < 0.05. Ctrl refers to control group.

Article Snippet: We obtained the Mouse Corticosterone (CORT) ELISA Kit (Elabscience, Shanghai, China), FITC—dextran (4 kD) (Sigma, Saint Louis, MI, USA); the mouse intestinal fatty acid-binding protein (FABP) ELISA Kit (CUSABIO, Wuhan, China); and all of the 11 SCFA standards (ZZ Standards Co., Ltd., Shanghai, China).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Staining, Control

Journal: iScience

Article Title: The mesenteric adipokine SFRP5 alleviated intestinal epithelial apoptosis improving barrier dysfunction in Crohn's disease

doi: 10.1016/j.isci.2024.111517

Figure Lengend Snippet: The adipokine SFRP5 was increased in the htMAT of CD patients (A) Representative image of IHC staining showing that SFRP5 expression was elevated in htMAT but was only visible in NL-MAT and nMAT. (B) Quantitative analysis of the results of the IHC staining for SFRP5. (C and D) The level of the SFRP5 protein in MAT was detected by WB. (E) The RT-qPCR result of SFRP5 in MAT. (F) The levels of SFRP5 in the serum of humans were determined by enzyme-linked immunosorbent assay (ELISA). CD, Crohn disease; NL, colon cancer; HC, healthy control; MAT, mesenteric adipose tissue; htMAT, hypertrophic MAT in patients with CD; nMAT, normal MAT in patients with CD; NL-MAT, normal MAT in patients with colon cancer. N = 18 per group. The data are presented as the mean ± SD. ANOVA (Tukey’s multiple test) was used for comparison of measurement data. ∗ p < 0.05. NS, not significant.

Article Snippet: ELISA kit (I-FABP) , BOSTER , EK1622.

Techniques: Immunohistochemistry, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control, Comparison

Journal: iScience

Article Title: The mesenteric adipokine SFRP5 alleviated intestinal epithelial apoptosis improving barrier dysfunction in Crohn's disease

doi: 10.1016/j.isci.2024.111517

Figure Lengend Snippet: The protective effect of SFRP5 on CD-like colitis in vivo may be at least partially mediated by Wnt5a/JNK signaling (A–D) The levels of Wnt5a, p-JNK, and Bcl2 in the colonic mucosa were analyzed by WB. (E and F) The levels of TNF-α and IL-6 were detected via ELISA. (G) DAI scores. (H and I) Representative images of H&E staining of the whole colon and colonic inflammation scores. (J and K) FD4 levels in the serum and colonic TEER values. (L–P) The ZO-1, Claudin-1, and C-caspase3 protein levels were detected by WB. WT, wild-type; TNBS, 2,4,6-trinitrobenzenesulfonic acid; SFRP5, rAAV9-SFRP5; DAI, disease activity index; FD4, fluorescein isothiocyanate-dextran (4 kDa); TEER, transepithelial electrical resistance; ZO-1, zonula occludens-1; C-caspase3, cleaved caspase3. N = 6 per group. The data are presented as the mean ± SD. ANOVA (Tukey’s multiple test) was used for comparison of measurement data. ∗ p < 0.05.

Article Snippet: ELISA kit (I-FABP) , BOSTER , EK1622.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Staining, Activity Assay, Comparison

Journal: iScience

Article Title: The mesenteric adipokine SFRP5 alleviated intestinal epithelial apoptosis improving barrier dysfunction in Crohn's disease

doi: 10.1016/j.isci.2024.111517

Figure Lengend Snippet:

Article Snippet: ELISA kit (I-FABP) , BOSTER , EK1622.

Techniques: Recombinant, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, In Situ, Software